Contact

wojciech.madry@pwr.edu.pl 
L-1, room 30
LinkedIn

Research

My research focuses on the investigation of proteolytic enzymes, primarily deubiquitinases such as the papain-like protease (PLpro) of SARS-CoV-2. I develop and validate chemical tools (e.g. fluorogenic substrates and activity-based probes) which are highly selective towards SARS-CoV-2 PLpro. Such tools make it possible to study the enzyme in detailed way and thereby to get a deeper understanding of its role during infection. By utilizing activity-based probes, I seek to develop an assay that will accelerate the screening of potential SARS-CoV-2 PLpro inhibitors.

This work combines many areas of chemistry and biology and thus I employ various techniques originating from these fields, including solid-phase peptide synthesis, enzyme kinetic studies, LC-MS analysis, cell-based assays, SDS-PAGE, western blotting and more.

Education and research internships

2025 – present PhD in Chemical sciences, Wroclaw University of Science and Technology, Faculty of Chemistry, Department of Chemical Biology and Bioimaging, Wrocław, Poland.

2024 – 2025 MSc in Biotechnology, Wroclaw University of Science and Technology, Faculty of Chemistry, Wrocław, Poland; Development of fluorogenic substrates for SARS-CoV-2 PLpro investigation.

2020 – 2024 BSc in Biotechnology, Wroclaw University of Science and Technology, Faculty of Chemistry, Wrocław, Poland; Fluorogenic substrates for bacterial serine protease activity investigation.

Conferences

Wojciech Mądry, Shaun K. Olsen, Wioletta Rut, A Positional Scanning Approach to Develop Selective Substrates for DUBs, Fourth Annual Meeting of the ProteoCure COST Action, Heraklion, Greece, 5/2025