Marcin Poręba

Publications (from ISI Master Journal List)

1. Poreba M, Protease-activated prodrugs: strategies, challenges and future directions FEBS Journal 2020, 1. View at Publisher
2. Poreba M, Groborz K, Navarro M, Snipas SJ, Drag M, Salvesen GS, Caspase selective reagents for diagnosing apoptotic mechanisms Cell Death and Differentiation 2019, 26, 229. View at Publisher
3. Cogo F, Poreba M, Rut W, Groborz K, Smyth P, Johnston MC, Williams R, Longley DB, Burden RE, Salvesen GS, Drag M, Scott Ch., Development of an advanced nanoformulation for the intracellular delivery of a caspase-3 selective activity-based probe Nanoscale 2019, 11, 742. View at Publisher
4. Poreba M, Rut W, Groborz K, Snipas SJ, Salvesen GS, Drag M, Potent and selective caspase-2 inhibitor prevents MDM-2 cleavage in reversine-treated colon cancer cells Cell Death and Differentiation 2019, 26, 2695. View at Publisher
5. Poreba M, Recent advances in the development of legumain-selective chemical probes and peptide prodrugs Biological Chemistry 2019, 1. View at Publisher
6. de Vries LE, Sanchez MI, Groborz K, Kuppens L, Poreba M, Lehmann C, Nevins N, Withers-Martinez C, Hirst DJ, Yuan F, Arastu-Kapur S, Horn M, Mares M, Bogyo M, Drag M, Deu E, Characterization of P. falciparum dipeptidyl aminopeptidase 3 specificity identifies differences in amino acid preferences between peptide-based substrates and covalent inhibitors FEBS Journal 2019, 286, 3998. View at Publisher
7. Groborz K, Gonzalez-Ramirez ML, Snipas SJ, Salvesen GS, Drag M, Poreba M, Exploring the prime site in caspases as a novel chemical strategy for understanding the mechanisms of cell death: a proof of concept study on necroptosis in cancer cells Cell Death and Differentiation 2019, 1. View at Publisher
8. Drąg-Zalesińska M, Rembiałkowska N, Borska S, Saczko J, Drąg M, Poręba M, Kulbacka J, A New Betulin Derivative Stimulates the Synthesis of Collagen in Human Fibroblasts Stronger than its Precursor In vivo 2019, 33, 1087. View at Publisher
9. Poreba M, Groborz K, Vizovisek M, Maruggi M, Turk D, Turk B, Powis G, Drag M, Salvesen GS, Fluorescent probes towards selective cathepsin B detection and visualization in cancer cells and patient samples Chemical Science 2019, 10, 8461. View at Publisher
10. Rut W, Nielsen NV, Czarna J, Poreba M, Kanse SM, Drag M, Fluorescent activity-based probe for the selective detection of Factor VII activating protease (FSAP) in human plasma Thrombosis Research 2019, 182, 124. View at Publisher
11. Poreba M, Groborz K, Rut W, Pore M, Snipas SJ, Vizovisek M, Turk B, Drag M, Salvesen GS, The Activome: multiplexed probing of activity of proteolytic enzymes using mass cytometry-compatible activity-based probes (TOF-probes) bioRxiv 2019, 1. View at Publisher
12. Lunde NN, Gregersen I, Ueland T, Shetelig C, Holm S, Kong XY, Michelsen AE, Otterdal K, Yndestad A, Broch K, Gullestad L, Nyman TA, Bendz B, Eritsland J, Hoffmann P, Skagen K, Gonçalves I, Nilsson J, Grenegard M, Poreba M, Drag M, Seljeflot I, Sporsheim B, Espevik T, Skjelland M, Johansen HT, Solberg R, Aukrust P, Björkbacka H, Andersen GO, Halvorsen B, Legumain is upregulated in acute cardiovascular events and associated with improved outcome - potentially related to anti-inflammatory effects on macrophages. Atherosclerosis 2019, 1. View at Publisher
13. Poreba M, Rut W, Vizovisek M, Groborz K, Kasperkiewicz P, Finlay D, Vuori K, Turk D, Turk B, Salvesen GS, Drag M, Selective imaging of cathepsin L in breast cancer by fluorescent activity-based probes Chemical Science 2018, 9, 2113. View at Publisher
14. Gonzalez-Ramirez ML*, Poreba M*, Snipas SJ, Groborz K, Drag M, Salvesen GS (* equal contribution), Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1 Journal of Biological Chemistry 2018, 293, 7058. View at Publisher
15. Poreba M, Kasperkiewicz P, Rut W, Drag M, Screening Combinatorial Peptide Libraries in Protease Inhibitor Drug Discovery Extracellular Targeting of Cell Signaling in Cancer: Strategies Directed at MET and RON Receptor Tyrosine Kinase Pathways, 1 2018, 1. View at Publisher
16. Rut W, Poreba M, Kasperkiewicz P, Snipas SJ, Drag M, Selective substrates and activity-based probes for imaging of the human constitutive 20S proteasome in cells and blood samples Journal of Medicinal Chemistry 2018, 61, 5222. View at Publisher
17. Kasperkiewicz P, Kołt S, Janiszewski T, Groborz K, Poręba M, Snipas SJ, Salvesen GS, Drąg M, Determination of extended substrate specificity of the MALT1 as a strategy for the design of potent substrates and activity-based probes Scientific Reports 2018, 8, 15998. View at Publisher
18. Balogh B, Dahmen M, Stahl M, Poreba M, Gersch M, Drag M, Sieber SA , Insights into ClpXP proteolysis: heterooligomerization and partial deactivation enhance chaperone affinity and substrate turnover in Listeria monocytogenes Chemical Science 2017, 8, 1592. View at Publisher
19. Rut W, Zhang L, Kasperkiewicz P, Poreba M, Hilgenfeld R, Drag M, Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 protease Antiviral Research 2017, 139, 88. View at Publisher
20. Kasperkiewicz P, Poreba M, Groborz K, Drag M, Emerging challenges in the design of selective protease substrates, inhibitors and activity-based probes for indistinguishable proteases FEBS Journal 2017, 284, 1518. View at Publisher
21. Drag-Zalesinska M, Drag M, Poreba M, Borska S, Kulbacka J, Saczko J, Anticancer properties of ester derivatives of betulin in human metastatic melanoma cells (Me-45) Cancer Cell International 2017, 17, 7. View at Publisher
22. Poreba M, Szalek A, Rut W, Kasperkiewicz P, Rutkowska-Wlodarczyk I, Snipas SJ, Itoh Y, Turk D, Turk B, Overall CM, Kaczmarek L, Salvesen GS, Drag M, Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families Scientific Reports 2017, 7, 43135. View at Publisher
23. Poreba M, Salvesen GS, Return of the Ice Age: Caspases Safeguard against Inflammatory Cell Death Cell Chemical Biology 2017, 24, 550. View at Publisher
24. Lunde NN, Haugen MH, Bodin Larsen KB, Damgaard I, Pettersen SJ, Kasem R, Rut W, Drag M, Poreba M, Johansen HT, Solberg R, Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions Biochimie 2017, 139, 27. View at Publisher
25. Poreba M, Salvesen GS, Drag M, Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity Nature Protocols 2017, 12, 2189. View at Publisher
26. Gersch M, Stahl M, Poreba M, Dahmen M, Dziedzic A, Drag M, Sieber SA, Barrel-shaped ClpP proteases display attenuated cleavage specificity ACS Chemical Biology 2016, 11, 389. View at Publisher
27. Poreba M, Solberg R, Rut W, Lunde NN, Kasperkiewicz P, Snipas SJ, Mihelic M, Turk D, Turk B, Salvesen GS, Drag M., Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes Cell Chemical Biology 2016, 23, 1023. View at Publisher
28. Rut, W.; Kasperkiewicz, P.; Byzia, A.; Poreba, M.; Groborz, K.; Drag, M., Recent advances and concepts in substrate specificity determination of proteases using tailored libraries of fluorogenic substrates with unnatural amino acids Biol. Chem. 2015, 396, 329. View at Publisher
29. Hachmann J, Edgington-Mitchell LE, Poreba M, Sanman LE, Drag M, Bogyo M, Salvesen GS., Probes to monitor activity of the paracaspase MALT1 Chemistry & biology 2015, 22, 139. View at Publisher
30. Drag-Zalesinska M, Wysocka T, Borska S, Drag M, Poreba M, Choromanska A, Kulbacka J, Saczko J., The new esters derivatives of betulin and betulinic acid in epidermoid squamous carcinoma treatment - In vitro studies. Biomedicine & Pharmacotherapy 2015, 72, 91. View at Publisher
31. Kasperkiewicz P, Poreba M, Snipas SJ, Lin SJ, Kirchhofer D, Salvesen GS, Drag M., Design of a Selective Substrate and Activity Based Probe for Human Neutrophil Serine Protease 4. PLoS One 2015, 10, 1. View at Publisher
32. Rajkovic, J.; Poreba, M.; Caglic, D.; Vidmar, R.; Wilk, A.; Borowik, A.; Salvesen, G. S.; Turk, V.; Drag, M.; Turk, B., Biochemical Characterization and Substrate Specificity of Autophagin-2 from the Parasite Trypanosoma cruzi J. Biol. Chem. 2015, 290, 28231. View at Publisher
33. Galiullina RA, Kasperkiewicz P, Chichkova NV, Szalek A, Serebryakova MV, Poreba M, Drag M, Vartapetian AB, Substrate Specificity and Possible Heterologous Targets of Phytaspase, a Plant Cell Death Protease. Journal of Biological Chemistry 2015, 290, 24806. View at Publisher
34. Poreba M, Szalek A, Kasperkiewicz P, Rut W, Salvesen GS, Drag M, Small Molecule Active Site Directed Tools for Studying Human Caspases Chemical Reviews 2015, 115, 12546. View at Publisher
35. Poręba, M.; Mihelic, M.; Krai, P.; Rajkovic, J.; Krężel, A.; Pawełczak, M.; Klemba, M.; Turk, D.; Turk, B.; Latajka, R.; Drąg, M., Unnatural amino acids increase activity and specificity of synthetic substrates for human and malarial cathepsin C. Amino Acids 2014, 46, 931. View at Publisher
36. Kasperkiewicz, P.; Poreba, M.; Snipas, S. J.; Parker, H.; Winterbourn, C. C.; Salvesen, G. S.; Drag, M., Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling. Proc. Natl. Acad. Sci. U. S. A. 2014, 111, 2518. View at Publisher
37. Poręba, M.; Szalek, A.; Kasperkiewicz, P.; Drąg, M., Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity. Methods Mol. Biol. 2014, 1133, 41. View at Publisher
38. Poreba, M.; Kasperkiewicz, P.; Snipas, S. J.; Fasci, D.; Salvesen, G. S.; Drąg, M., Unnatural amino acids increase sensitivity and provide for the design of highly selective caspase substrates Cell Death & Differentiation 2014, 21, 1482. View at Publisher
39. Węglarz-Tomczak, E.; Poręba, M.; Byzia, A.; Berlicki, Ł.; Nocek, B.; Mulligan, R.; Joachimiak, A.; Drąg, M.; Mucha, A., An integrated approach to the ligand binding specificity of Neisseria meningitidis M1 alanine aminopeptidase by fluorogenic substrate profiling, inhibitory studies and molecular modeling. Biochimie 2013, 95, 419. View at Publisher
40. Carroll, R.K.; Veillard, F.; Gagne, D.T.; Lindenmuth, J.M.; Poreba, M.; Drag, M.; Potempa, J.; Shaw, L.N., The Staphylococcus aureus leucine aminopeptidase LAP is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine. Biol. Chem. 2013, 394, 791. View at Publisher
41. Zeiler, E.; List, A.; Alte, F.; Gersch, M.; Wachtel, R.; Poręba, M.; Drąg, M.; Groll, M.; Sieber, S.A., Structural and functional insights into caseinolytic proteases reveal an unprecedented regulation principle of their catalytic triad. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 11302. View at Publisher
42. Poręba, M.; Stróżyk, A.; Salvesen, G.S.; Drag, M. , Caspase Substrates and Inhibitors. Cold Spring Harb. Perspect. Biol. 2013, 5, 1. View at Publisher
43. Poreba, M.; McGowan, S.; Skinner-Adams, T. S.; Trenholme, K. R.; Gardiner, D. L.; Whisstock, J. C.; To, J.; Salvesen, G. S.; Dalton, J. P.; Drąg, M., Fingerprinting the Substrate Specificity of M1 and M17 Aminopeptidases of Human Malaria, Plasmodium Falciparum. PLoS One 2012, 7, 31938. View at Publisher
44. Hachman, J.; Snipas, S. J.; Van Raam, B. J.; Cancino, E. M.; Houlihan, E. J.; Poręba, M.; Kasperkiewicz, P.; Drąg, M.; Salvesen, G. S., Mechanism and specificity of the human paracaspase MALT1. Biochem. J. 2012, 443, 287. View at Publisher
45. Poręba, M.; Gajda, A.; Picha, J.; Jiracek, J.; Marschner, A.; Klein, C.; Salvesen, G. S.; Drąg, M., S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors. Biochimie 2012, 94, 704. View at Publisher
46. Sivaraman, K.K.; Oellig, C.A.; Huynh, K.; Atkinson, S.C.; Poreba, M.; Perugini, M.A.; Trenholme, K.R.; Gardiner, D.L.; Salvesen, G.; Drag, M.; Dalton, J.P.; Whisstock, J.C.; McGowan, S., X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase PfM18AAP. J. Mol. Biol. 2012, 422, 495. View at Publisher
47. Veillard, F.; Potempa, B.; Poreba, M.; Drag, M.; Potempa, J., Gingipain aminopeptidase activities in Porphyromonas gingivalis. Biol. Chem. 2012, 393, 1471. View at Publisher
48. Zervoudi, E.; Papakyriakou, A.; Georgiadou, D.; Evnouchidou, I.; Gajda, A.; Poręba, M.; Salvesen, G. S.; Drąg, M.; Hattori, A.; Swevers, L.; Vourloumis, D.; Stratikos, E., Probing the S1 specificity pocket of the aminopeptidases that generate antigenic peptides. Biochem J. 2011, 435, 411. View at Publisher
49. Poręba, M.; Drąg, M., Current strategies for probing substrate specificity of proteases. Curr. Med. Chem. 2010, 17, 3968. View at Publisher

Publications (in polish)

1. Poreba, M.; Kaspazy inicjatorowe i egzekucyjne w kontekście projektowania małocząsteczkowych markerów chemicznych Nowe trendy w naukach przyrodniczych – monografie. 2012, tom V, 141.
2. Barwinek, D.; Poreba, M.; Drag. M; Substraty fluorogeniczne zawierające nienaturalne aminokwasy jako narzędzie w badaniu kaspaz. Prace Badawcze Studentów Politechniki Wrocławskiej, 2012, 10, 13.
3. Poreba, M.; Kasperkiewicz, P., Narzędzia chemii biologicznej w badaniu proteaz. Nowe trendy w naukach przyrodniczych – monografie. 2012, tom II, 112.
4. Kasperkiewicz, P.; Poreba, M., Ludzka neutrofilowa elastaza (HNE) – przegląd. Nowe trendy w naukach przyrodniczych – monografie. 2012, tom II, 187.